Coupling of Fiber Optic Cable and excitation Light Source Not all light from a light source can travel within the fiber core. If the divergence angle of the light source is too large, it is impossible to form full emission at the fiber boundary, and the light will be scattered through the boundary between the core and the cladding. How to efficiently couple the excitation light power of the LED into the Y-type fiber needs to consider two variables: the luminous size, radiant intensity and power distribution of the LED, and the core diameter, numerical aperture and refractive index of the fiber. In PCR applications, for example, one end of the Y-shaped Fiber Optic Bundle is the common end of the self-aggregating optical fiber composed of two bundles of optical fibers, and the other end is divided into two branches, which are used as the light source optical fiber and the receiving optical fiber respectively, with solid durability, high stability and insertion loss. and low reflection loss, thereby greatly reducing the loss of light energy, enhancing the ability to collect light, and improving the accuracy and reliability of signal detection. Hecho adopts the SCHOTT glass fiber imported from Germany, which has low transmission loss, good flexibility, and can be bent at will. The monofilament is slender and easy to distribute and combine. It provides customized Optical Fiber with various core diameters, lengths and different structures to meet the needs of different customers differenet requirements.

The Fiber Optic system used in the PCR instrument includes excitation light source, excitation light path, fluorescence collection and detection light path, and it is the core module that determines the weak fluorescence detection capability of the system. Usually, the intensity of the excitation light is more than 1000 times of the fluorescence intensity. Therefore, the detection of the fluorescent signal in the PCR instrument belongs to the acquisition of the weak signal under the strong background signal. In the traditional oblique, transmissive and confocal optical system architecture modules, various optical components such as lenses, mirrors, and prisms are integrated, which requires a certain mechanical structure to assist in fixing, which increases the difficulty of processing, and reduced instrument stability. In addition, the traditional optical system is to irradiate excitation light into the reaction sample in the sample cell from the upper part of the base, and then receive the fluorescence instantaneously excited by the reaction tube from the upper part of the base to realize detection. In this process of excitation light transmission and emission fluorescence reception, the system has a large loss of light energy, poor light collection ability, and poor transmission efficiency, resulting in a decrease in fluorescence detection sensitivity. In order to maximize the optical signal throughput and reduce the transmission loss, we need to choose a Fiber Optic Bundle system that is different from the traditional combined lens type.

Entering winter, as the temperature dropped and Omicron spread throughout the world, the epidemic broke out again in many countries. Christmas is approaching, crowd gatherings will increase the risk of infection, and we once again call on everyone to do a good job of protection when going out and gatherings. Since the outbreak of the new crown epidemic, Hecho has provided more than 5 million pcs customized PCR Fiber Optic Cable and POCT Optical Fiber Cable to IVD companies in the industry for the detection of COVID-19. With excellent product performance and stable and fast delivery capabilities, it has obtained Unanimous praise from the market. When the epidemic will end, we still need to work together globally to fight the epidemic, and we look forward to this day as soon as possible. In the past days, gaining your trust and support is our most precious asset and the most precious gift you have given us. To this end, on the occasion of the arrival of this new year, we hereby express our warmest thanks and best regards to you! I wish you all a joyous and wonderful Christmas. Good health and good luck in the new year.

As all kinds of electronic products tend to be miniaturized, the application of traditional welding technology in various new electronic components has a certain test. In order to meet such market demand, in the welding technology, it can be said that the technology is constantly improving, and the welding way is more diversified. The Light Source for laser welding is a laser light-emitting diode, which can be focused on the solder spot by an optical system. The advantage of laser welding is that it can precisely control and optimize the energy required for welding. It is suitable for selective reflow welding process or using tin wire connector. For SMD components, the solder paste should be applied first and then welded. The welding process is divided into two steps: first the solder paste is heated, and the solder joint is preheated. After that, the solder paste used for welding is completely melted, and the solder is completely wetted on the pad to form the weld. Use laser generator and optical focusing component welding, energy density, high heat transfer efficiency, non-contact welding, solder can be solder paste or tin wire, especially suitable for welding small space solder spot or small solder spot power is small, save energy. Laser welding features: ● Multi-axis servo motor board card control, high positioning accuracy ● Small laser spot, in the small size of pads, spacing devices have obvious welding advantages ● Non-contact welding, no mechanical stress, static risk ● Wuxi slag, reduce flux waste, low production cost ● Weldable products are rich in type ● Choice of solder Advantages of laser welding: For ultra-fine electronic substrate, multi-layer electronic components, "traditional technology" has been unable to apply, which prompted the rapid progress of technology. The processing of ultra-small parts that are not suitable for the traditional soldering iron method is finally completed by laser welding. Non - contact welding is the biggest advantage of laser welding. There is no need to touch the substrate and electronic components at all, and providing solder only by laser irradiation does not pose a physical burden. Effective heating with a blue laser beam is also an advantage, which can be used to illuminate narrow areas where the soldering head cannot enter and at different angles when there is no distance between adjacent components in a dense assembly. The soldering iron head needs to be replaced regularly, while the spare parts for laser welding need to be replaced are very few and the maintenance cost is low. Hecho Technology supply the Fiber Optic Bundles for Laser selective soldering, customized service is available to meet the different design requirements of customers.

Dear Customers, Sincerely thanks for your always support. The traditional Mid-Autumn Festival and China National Day is coming, wish you all a happy holiday. Pls note that Hecho Fiber Optics will have couple days holiday for the two holiday: Mid-Autumn Festival: from Sep. 19th to Sep. 21st National Day: from Oct. 1st to Oct. 7th If there's any new questions about the Hecho Fiber Optic Bundles & Fiber Optic Illuminator, pls send E-mail to owen@gohecho.cn or any emergency pls call to +86 133 8200 9944. Keep Social distancing, pay attention to personal protection for the COVID-19. Thanks & best regards, Nanjing Hecho Technology Co., Ltd 2021-9-8

The difference between quantitative real-time PCR (qPCR) and end-point PCR is the step at which the PCR product amount is measured. In traditional PCR, product quantitation is performed once at the end of the reaction. In qPCR, the amount of PCR product is measured after every amplification round. qPCR instruments are designed for both PCR cycling and the detection of signal from fluorescent dyes during amplification. By monitoring the progress of amplification in real time, the point in time when the amplification of the target is detected above background can be identified. The fewer targets present in the reaction, the later the first significant signal is detected. This technique, therefore, is of great advantage when gene-expression differences are analyzed. There are two ways to detect PCR signal with the help of fluorescent dyes: fluorescent dyes that nonspecifically bind to double-stranded DNA (Dye-based qPCR) or fluorescently labeled, sequence-specific probes (Probe-based qPCR). In Dye-based qPCR the use of universal fluorescent DNA-intercataling dyes enables analysis of many targets without having to synthesize sequence-specific probes. However, this method has the disadvantage of detecting all specific and unspecific amplification events as well as primer–dimers because the dye binds to all double-stranded DNA. To achieving high PCR specificity, avoiding background and primer–dimer formation is critical. Probe-based qPCR method is more precise, as the use of sequence-specific fluorescently labeled probes ensures the detection of the target product only. A variety of probe fluorescence chemistry is currently available. Multiple fluorescent probes can be used in multiplex qPCR, although the use of specific probes is more expensive than using DNA-intercalating dyes. qRT-PCR One Step Kits allow reverse transcription and real-time PCR to be performed in one step. In this case, RNA is the template for qPCR. One-step qPCR kits include separate qPCR and reverse transcription master mixes. Same like qPCR, the qRT-PCR One Step Kits are also available in both Dye-based and Probe-based formats. Many real-time PCR instruments available on the market require a passive internal reference dye (either ROX™ or fluorescein) for normalization of the fluorescent signal. The use of passive reference dyes does not interfere with the qPCR. The reference dyes are either supplied separately, or they can be included in the qPCR mastermix at appropriate concentration. biotechrabbit offers both. The choice depends on your instrument requirements for reference dye.

Nanjing Hecho Technology is a leading provider of Fiber Optic solutions in China since 2004. By providing innovative technology and product solutions to customers in multiple countries around the world, companies and customers can enjoy the value of innovation. Products including Fiber Optic Bundle, Fiber Optic Illuminator, Fiber Optic Sensor, and so on, flexible to meet the diverse needs of customers in different industries, such as industry, medical, lighting, optics, and the pursuit of rapid innovation. The Laser and Photonics Exhibition will be held from March 17th to 19th, 2021. Our booth number is W5.5155. Welcome every customers to visit our booth!

Dear valued customers, 2020 was an extraordinary year, People all over the world are still suffering from the epidemic COVID-19. I hope we can win the fight against the epidemic soon. In order to better serve your company, we hereby inform you of our company's holiday time for the Chinese New Year in 2021, so as to make good preparations for the holidays. Thanks for your support! Pls note that Hecho will be closed for the CNY celebration from Feb. 10th 2021 to Feb. 19th 2021. Normal business will resume on Feb. 20th 2021. During the holiday period, there will likely be a delay in reply to your email or phone call, we apologize for the delay and any inconvenience this may cause. If something urgent comes up or you need any immediate help, pls feel free ti reach out to us via email and call. If there's any questions on Fiber Optic Illuminators or Fiber Optic Bundles and Fiber Optic Cables, pls feel free to contact us. Best Wishes. Nanjing Hecho Technology Co., Ltd 2021-2-4

Analytica China in Munich is Asia's largest international expo in the field of analysis and biochemical technology. It is a platform for outstanding companies in the industry to comprehensively display new technologies, products and solutions. Bringing together industry enterprises, showing front-end technology and high-end products, is the vane of industry development. analytica China will comprehensively cover and display various fields of laboratory research and industry development: Life Science, Biotechnology and Diagnosis Analysis and quality control Laboratory equipment and technology Food safety equipment and technology Environmental monitoring equipment and technology Due to the sudden outbreak of the new crown virus, the biochemical analysis industry market is extremely hot. Taking this opportunity, Nanjing Hongzhao Technology Co., Ltd. will exhibit a variety of High transfer rate and Low CV value Fiber Optic Bundles, PCR Fiber Optic Cables that can be used in nucleic acid detection equipment. Welcome new and old customers of Everbright to visit and guide. Exhibition time: November 16-18, 2020 Pavilion: Shanghai New International Expo Center Booth number: E5. 5211

Dear customers, According to the national statutory holiday regulations and combined with the actual situation of the company, the arrangements for the National Day and Mid-Autumn Festival holidays in 2020 are as follows: 1. There will be a holiday from October 1 to 8, 2020, for a total of 8 days. Work on October 9 (Friday). 2. All employees of the company are requested to make their own pre-holiday work arrangements, and check related facilities and equipment, do a good job in fire prevention and theft, and ensure the safety and order of the workshop and office. 3. The employees of the company should maintain smooth communication during the holidays to meet the needs of the company's work. During the holiday season, everyone pays attention to safety. The epidemic is not over yet, so you must wear a mask when you go out. 4. I wish our customers and all employees a happy holiday and good health in advance! If there's any requirements for the Fiber Optic Illuminators, Fiber Optic Bundles, and other Fiber Optic products in this period, pls E-mail to: owen@gohecho.cn. NANJING HECHO TECHNOLOGY CO., LTD. 2020-9-28

Nanjing Hecho Technology is a leading provider of Fiber Optic solutions in China since 2004. By providing innovative technology and product solutions to customers in multiple countries around the world, companies and customers can enjoy the value of innovation. Products including Fiber Optic Bundles, Fiber Optic Illuminators, Fiber Optic Sensors, Machine Vision Lighting and so on, flexible to meet the diverse needs of customers in different industries, such as industry, medical, lighting, optics, and the pursuit of rapid innovation. Affected by the COVID-19, The CIOE exhibition will be held from September 9th to 11th, 2020. Our booth number is 5E50. Welcome every customers to visit our booth!

LASER WORLD OF PHOTONICS CHINA presents the full range of product content of the optoelectronic industry from an international perspective, designed to meet the unique needs of the Chinese market. As the Asian laser, optics, and optoelectronics industry event, from basic research to industrial applications, the Munich Shanghai Optical Expo leads the industry development trend, focusing on covering lasers and optoelectronics, optical and optical manufacturing, laser production and processing technology, imaging, inspection and quality control The comprehensive product content of the four major sectors is an industry exhibition not to be missed. Affected by this year's new coronavirus, this Expo will be postponed to July 3 to 5, Nanjing Hecho Technology Co., Ltd. will continue to participate in this Shanghai Expo in Munich, welcome friends to visit. In this exhibition, Nanjing Hecho Technology will exhibit traditional superior products such as Fiber Optic Bundles, near-infrared optical fiber light guide, Fiber Optic Illuminator, including Halogen light source, LED light source, etc. As an authorized agent of SCHOTT Group, our company will also exhibit SCHOTT cold light sources and fiber optic bundles. In addition, the company's newly developed high-power, ultra-high brightness LED cold light source will have the opportunity to exhibit. Nanjing Hecho Technology will provide you with more professional optical fiber transmission solutions. Our booth is at 7.1A 143. Welcome!